SHORT COMMUNICATION A Portable Allosteric Mechanism
نویسندگان
چکیده
Theallostericmechanismbywhich the gene expression regulatory protein AraC regulates its DNA-binding activity is shown to be portable by grafting it to -galactosidase, generating an arabinose-regulated -galactosidase. A portion of the -peptide sequence that complements the activity of -acceptor -galactosidase was inserted into a nonessential region of the regulatory peptidyl arm of AraC protein. Arabinose, which regulates the position of the arm in AraC protein now regulates the availability of the -peptide to -acceptor -galactosidase, thereby modulating its activity in response to arabinose. Proteins 2004;57:9–11. © 2004Wiley-Liss, Inc.
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